When constructing fusion proteins, it is necessary to make sure that stop codons only appear at the end of the DNA sequence so that translation of the resulting. As part of the International Genetically Engineered Machine (iGEM) competition, our team is investigating new methods of increasing the efficiency of. Guide. DNA ; mRNA; Protein Structure. SynthetiQ. Who and Why? How? Dance Your PhD Queen's University. Gold Sponsor(s). New England Biolabs Canada.